pH-Xtra™
  • Luxcel's easy-to-use pH-Xtra Glycolysis Assay, is a highly flexible 96 or 384-well fluorescence plate reader- based approach for the direct, real-time, kinetic analysis of extracellular acidification rates (ECA/ECAR) to assess glycolytic function in cells. Glycolysis, a key energy-producing pathway, is involved in a wide array of physiological and pathophysiological processes, including cellular adaptation to hypoxia and ischemia, and the development and progression of tumorigensis.

     

    Luxcel's flexible plate reader format, allows multiparametric or multiplex combination with Luxcel's other products, as well as combination with commonly available reagents to measure oxygen consumption, LDH, JC-1, MMP ( ), ROS, and cellular ATP. For example, pH-Xtra in combination with Luxcel's MitoXpress® Xtra Oxygen Consumption Assay (HS Method (Cat No. MX-200) allows the simultaneous real-time measurement of mitochondrial respiration and glycolysis and analysis of the metabolic phenotype of cells and the shift (flux) between the two pathways under pathological states.

     

    Product: pH-Xtra™ Glycolysis Assay

                      pH-Xtra™ Reagent

     

                      Respiration Buffer

     

    Product

    Description

    Assays

    Cat. No.

    pH-Xtra™ Glycolysis Assay

     

    Plate-based measurement of Extracellular Acidification (ECA). For cell-based and 3D culture applications.

    Kit Content:

    ·          1 vial pH-Xtra™ reagent [Cat No. PH-100]

    ·          1 tablet Respiration Buffer [Cat No. RB-100]

    ·          1 User Manual

    ~100 assays

    PH-200

    pH-Xtra™ Glycolysis Assay

    Multipack

     

    Plate-based measurement of Extracellular Acidification (ECA). For cell-based and 3D culture applications.

    Kit Content:

    ·          4 vials pH-Xtra™ reagent [Cat No. PH-100]

    ·          4 tablets Respiration Buffer [Cat No. RB-100]

    ·          1 User Manual

    ~400 assays

    PH-200/4

    pH-Xtra™ reagent

     

    Lyophilised water soluble probe for the measurement of Extracellular Acidification (ECA) or pH.

    Content:

    ·          1 vial of [H+] pH-sensitive probe

    ~100 assays

    PH-100

    Respiration Buffer

     

    Low phosphate-buffered media,  for the measurement of extracellular acidification (ECAR)

     

    Content:

    ·          1 tablet Respiration Buffer (provides 50ml 1x buffer)

    ~100 assays 

    RB-100